other:
Article Title: Corn plant event MON87460 and compositions and methods for detection thereof
Article Snippet: This digested and purified genomic DNA was used for ligation into the Lambda DASH® II/EcoRI Vector Kit (Stratagene).
Clone Assay:Article Title: Regulation of 7S globulin gene expression in zygotic and somatic embryos of oil palm.
Article Snippet: We describe here the characterization and expression analysis accumulation in somatic embryos is mirrored by a low accuof the oil palm GLO7A gene encoding a 7S globulin protein. mulation of the GLO7A mRNA.. The in vitro production of 7S globulins (and more generally salt-soluble proteins) is imPrevious work carried out in our laboratory showed that 7S proved by the addition to the culture medium of arginine, globulins accumulate in the oil palm zygotic embryo mostly between the 14 and 17 weeks after pollination.. To investigate sucrose and ABA, the effects of these 3 components being additive.
Isolation:Article Title: Regulation of 7S globulin gene expression in zygotic and somatic embryos of oil palm.
Article Snippet: We describe here the characterization and expression analysis accumulation in somatic embryos is mirrored by a low accuof the oil palm GLO7A gene encoding a 7S globulin protein. mulation of the GLO7A mRNA.. The in vitro production of 7S globulins (and more generally salt-soluble proteins) is imPrevious work carried out in our laboratory showed that 7S proved by the addition to the culture medium of arginine, globulins accumulate in the oil palm zygotic embryo mostly between the 14 and 17 weeks after pollination.. To investigate sucrose and ABA, the effects of these 3 components being additive.
Sequencing:Article Title: Regulation of 7S globulin gene expression in zygotic and somatic embryos of oil palm.
Article Snippet: We describe here the characterization and expression analysis accumulation in somatic embryos is mirrored by a low accuof the oil palm GLO7A gene encoding a 7S globulin protein. mulation of the GLO7A mRNA.. The in vitro production of 7S globulins (and more generally salt-soluble proteins) is imPrevious work carried out in our laboratory showed that 7S proved by the addition to the culture medium of arginine, globulins accumulate in the oil palm zygotic embryo mostly between the 14 and 17 weeks after pollination.. To investigate sucrose and ABA, the effects of these 3 components being additive.
cDNA Library Assay:Article Title: Regulation of 7S globulin gene expression in zygotic and somatic embryos of oil palm.
Article Snippet: We describe here the characterization and expression analysis accumulation in somatic embryos is mirrored by a low accuof the oil palm GLO7A gene encoding a 7S globulin protein. mulation of the GLO7A mRNA.. The in vitro production of 7S globulins (and more generally salt-soluble proteins) is imPrevious work carried out in our laboratory showed that 7S proved by the addition to the culture medium of arginine, globulins accumulate in the oil palm zygotic embryo mostly between the 14 and 17 weeks after pollination.. To investigate sucrose and ABA, the effects of these 3 components being additive.
Article Title: Monoterpene glycosyltransferase originating from hop and method for using same
Article Snippet: From immature and mature cones and leaves of Humulus lupulus (Shinshu wase variety), RNA was extracted with a Spectrum Plant Total RNA Kit (SIGMA), followed by treatment with an Oligotex-dT30 (SEQ ID NO: 21) mRNA purification kit (Takara Bio Inc., Japan) to obtain polyA(+) RNA. .. This polyA(+) RNA (5 μg) was used as a template to prepare a cDNA library using a Lambda ZAP cDNA synthesis kit (cDNA synthesis kit/Uni ZAP XR vector kit/GigaPacl III Gold Packaging Extract (Agilent)) in accordance with the method recommend by the manufacturer. ..
Construct:Article Title: Regulation of 7S globulin gene expression in zygotic and somatic embryos of oil palm.
Article Snippet: We describe here the characterization and expression analysis accumulation in somatic embryos is mirrored by a low accuof the oil palm GLO7A gene encoding a 7S globulin protein. mulation of the GLO7A mRNA.. The in vitro production of 7S globulins (and more generally salt-soluble proteins) is imPrevious work carried out in our laboratory showed that 7S proved by the addition to the culture medium of arginine, globulins accumulate in the oil palm zygotic embryo mostly between the 14 and 17 weeks after pollination.. To investigate sucrose and ABA, the effects of these 3 components being additive.
Cloning:Article Title: Vectors for production of antibodies
Article Snippet: .. Bp 717-673 Bp 3745-4184 Multiple cloning site from pBluescriptII sk(−), from the XhoI site bp 672-235 Bp 4190-4259 70 bp of IS10 from Tn10 Bp 4260-4301 Lambda DNA from pNK2859 Bp 4302-5167 Non-coding DNA from pNK2859 Bp 5168-7368 pBluescriptII sk(−) base vector (Stratagene, INC) bp 761-2961 SEQ ID NO: 2 X-MCS (Vector #5005) pTNMCS (Base Vector) with MCS Extension Bp 1-132 Remainder of F1 (−) ori of pBluescriptII sk(−) (Stratagene) Bp 4-135 Bp 133-1785 CMV Promoter/Enhancer from vector pGWIZ (Gene Therapy Systems) Bp 1786-3018 Transposase, modified from Tn10 (GeneBank accession #J01829) Bp 81-1313 Bp 3019-3021 Engineered stop codon Bp 3022-3374 Non-coding DNA from vector pNK2859 Bp 3375-3416 Lambda DNA from pNK2859 Bp 3417-3486 70 bp of IS10 left from Tn10 (GeneBank accession #J01829 Bp 1-70) Bp 3487-3704 Multiple cloning site from pBluescriptII sk(−), thru XmaI Bp 3705-3749 Multiple cloning site from pBluescriptII sk(−), from XmaI thru XhoI Bp 3750-3845 Multiple cloning site extension from XhoI thru PspOMI BP 3846-4275 Multiple cloning site from pBluescriptII sk(−), from PspOMI Bp 4276-4345 70 bp of IS10 from Tn10 (GeneBank accession #J01829 Bp 70-1) Bp 4346-4387 Lambda DNA from pNK2859 Bp 4388-5254 Non-coding DNA from pNK2859 Bp 5255-7455 pBluescriptII sk(−) base vector (Stratagene, INC) Bp 761-2961 SEQ ID NO: 3 HS4 Flanked BV (Vector #5006) Bp 1-132 Remainder of F1 (−) ori of pBluescriptII sk(−) (Stratagene) Bp 4-135 Bp 133-1785 CMV Promoter/Enhancer from vector pGWIZ (Gene Therapy Systems) Bp 229-1873, including the combination of 2 NruI cut sites Bp 1786-3018 Transposase, modified from Tn10 (GeneBank accession #J01829) Bp 81-1313 Bp 3019-3021 Engineered stop codon Bp 3022-3374 Non-coding DNA from vector pNK2859 Bp 3375-3416 Lambda DNA from pNK2859 Bp 3417-3490 70 bp of IS10 left from Tn10 (GeneBank accession #J01829 Bp 1-70) Bp 3491-3680 Multiple cloning site from pBluescriptII sk(−), thru NotI Bp 926-737 Bp 3681-4922 HS4—Beta-globin Insulator Element from Chicken gDNA Bp 4923-5018 Multiple cloning site extension XhoI thru MluI Bp 5019-6272 HS4—Beta-globin Insulator Element from Chicken gDNA Bp 6273-6342 70 bp of IS10 from Tn10 (GeneBank accession #J01829 Bp 70-1) Bp 6343-6389 Lambda DNA from pNK2859 Bp 6390-8590 pBluescriptII sk(−) base vector (Stratagene, INC) Bp 761-2961 SEQ ID NO: 4 K Tn-10 HS4 Flanked Backbone (Vector #5012) Bp. .. 1-132 Remaining of F1 (−) Ori from pBluescript II sk(−) (Stratagene Bp 4-135).
Lambda DNA Preparation:Article Title: Vectors for production of antibodies
Article Snippet: .. Bp 717-673 Bp 3745-4184 Multiple cloning site from pBluescriptII sk(−), from the XhoI site bp 672-235 Bp 4190-4259 70 bp of IS10 from Tn10 Bp 4260-4301 Lambda DNA from pNK2859 Bp 4302-5167 Non-coding DNA from pNK2859 Bp 5168-7368 pBluescriptII sk(−) base vector (Stratagene, INC) bp 761-2961 SEQ ID NO: 2 X-MCS (Vector #5005) pTNMCS (Base Vector) with MCS Extension Bp 1-132 Remainder of F1 (−) ori of pBluescriptII sk(−) (Stratagene) Bp 4-135 Bp 133-1785 CMV Promoter/Enhancer from vector pGWIZ (Gene Therapy Systems) Bp 1786-3018 Transposase, modified from Tn10 (GeneBank accession #J01829) Bp 81-1313 Bp 3019-3021 Engineered stop codon Bp 3022-3374 Non-coding DNA from vector pNK2859 Bp 3375-3416 Lambda DNA from pNK2859 Bp 3417-3486 70 bp of IS10 left from Tn10 (GeneBank accession #J01829 Bp 1-70) Bp 3487-3704 Multiple cloning site from pBluescriptII sk(−), thru XmaI Bp 3705-3749 Multiple cloning site from pBluescriptII sk(−), from XmaI thru XhoI Bp 3750-3845 Multiple cloning site extension from XhoI thru PspOMI BP 3846-4275 Multiple cloning site from pBluescriptII sk(−), from PspOMI Bp 4276-4345 70 bp of IS10 from Tn10 (GeneBank accession #J01829 Bp 70-1) Bp 4346-4387 Lambda DNA from pNK2859 Bp 4388-5254 Non-coding DNA from pNK2859 Bp 5255-7455 pBluescriptII sk(−) base vector (Stratagene, INC) Bp 761-2961 SEQ ID NO: 3 HS4 Flanked BV (Vector #5006) Bp 1-132 Remainder of F1 (−) ori of pBluescriptII sk(−) (Stratagene) Bp 4-135 Bp 133-1785 CMV Promoter/Enhancer from vector pGWIZ (Gene Therapy Systems) Bp 229-1873, including the combination of 2 NruI cut sites Bp 1786-3018 Transposase, modified from Tn10 (GeneBank accession #J01829) Bp 81-1313 Bp 3019-3021 Engineered stop codon Bp 3022-3374 Non-coding DNA from vector pNK2859 Bp 3375-3416 Lambda DNA from pNK2859 Bp 3417-3490 70 bp of IS10 left from Tn10 (GeneBank accession #J01829 Bp 1-70) Bp 3491-3680 Multiple cloning site from pBluescriptII sk(−), thru NotI Bp 926-737 Bp 3681-4922 HS4—Beta-globin Insulator Element from Chicken gDNA Bp 4923-5018 Multiple cloning site extension XhoI thru MluI Bp 5019-6272 HS4—Beta-globin Insulator Element from Chicken gDNA Bp 6273-6342 70 bp of IS10 from Tn10 (GeneBank accession #J01829 Bp 70-1) Bp 6343-6389 Lambda DNA from pNK2859 Bp 6390-8590 pBluescriptII sk(−) base vector (Stratagene, INC) Bp 761-2961 SEQ ID NO: 4 K Tn-10 HS4 Flanked Backbone (Vector #5012) Bp. .. 1-132 Remaining of F1 (−) Ori from pBluescript II sk(−) (Stratagene Bp 4-135).
Modification:Article Title: Vectors for production of antibodies
Article Snippet: .. Bp 717-673 Bp 3745-4184 Multiple cloning site from pBluescriptII sk(−), from the XhoI site bp 672-235 Bp 4190-4259 70 bp of IS10 from Tn10 Bp 4260-4301 Lambda DNA from pNK2859 Bp 4302-5167 Non-coding DNA from pNK2859 Bp 5168-7368 pBluescriptII sk(−) base vector (Stratagene, INC) bp 761-2961 SEQ ID NO: 2 X-MCS (Vector #5005) pTNMCS (Base Vector) with MCS Extension Bp 1-132 Remainder of F1 (−) ori of pBluescriptII sk(−) (Stratagene) Bp 4-135 Bp 133-1785 CMV Promoter/Enhancer from vector pGWIZ (Gene Therapy Systems) Bp 1786-3018 Transposase, modified from Tn10 (GeneBank accession #J01829) Bp 81-1313 Bp 3019-3021 Engineered stop codon Bp 3022-3374 Non-coding DNA from vector pNK2859 Bp 3375-3416 Lambda DNA from pNK2859 Bp 3417-3486 70 bp of IS10 left from Tn10 (GeneBank accession #J01829 Bp 1-70) Bp 3487-3704 Multiple cloning site from pBluescriptII sk(−), thru XmaI Bp 3705-3749 Multiple cloning site from pBluescriptII sk(−), from XmaI thru XhoI Bp 3750-3845 Multiple cloning site extension from XhoI thru PspOMI BP 3846-4275 Multiple cloning site from pBluescriptII sk(−), from PspOMI Bp 4276-4345 70 bp of IS10 from Tn10 (GeneBank accession #J01829 Bp 70-1) Bp 4346-4387 Lambda DNA from pNK2859 Bp 4388-5254 Non-coding DNA from pNK2859 Bp 5255-7455 pBluescriptII sk(−) base vector (Stratagene, INC) Bp 761-2961 SEQ ID NO: 3 HS4 Flanked BV (Vector #5006) Bp 1-132 Remainder of F1 (−) ori of pBluescriptII sk(−) (Stratagene) Bp 4-135 Bp 133-1785 CMV Promoter/Enhancer from vector pGWIZ (Gene Therapy Systems) Bp 229-1873, including the combination of 2 NruI cut sites Bp 1786-3018 Transposase, modified from Tn10 (GeneBank accession #J01829) Bp 81-1313 Bp 3019-3021 Engineered stop codon Bp 3022-3374 Non-coding DNA from vector pNK2859 Bp 3375-3416 Lambda DNA from pNK2859 Bp 3417-3490 70 bp of IS10 left from Tn10 (GeneBank accession #J01829 Bp 1-70) Bp 3491-3680 Multiple cloning site from pBluescriptII sk(−), thru NotI Bp 926-737 Bp 3681-4922 HS4—Beta-globin Insulator Element from Chicken gDNA Bp 4923-5018 Multiple cloning site extension XhoI thru MluI Bp 5019-6272 HS4—Beta-globin Insulator Element from Chicken gDNA Bp 6273-6342 70 bp of IS10 from Tn10 (GeneBank accession #J01829 Bp 70-1) Bp 6343-6389 Lambda DNA from pNK2859 Bp 6390-8590 pBluescriptII sk(−) base vector (Stratagene, INC) Bp 761-2961 SEQ ID NO: 4 K Tn-10 HS4 Flanked Backbone (Vector #5012) Bp. .. 1-132 Remaining of F1 (−) Ori from pBluescript II sk(−) (Stratagene Bp 4-135).
cDNA Synthesis:Article Title: Monoterpene glycosyltransferase originating from hop and method for using same
Article Snippet: From immature and mature cones and leaves of Humulus lupulus (Shinshu wase variety), RNA was extracted with a Spectrum Plant Total RNA Kit (SIGMA), followed by treatment with an Oligotex-dT30 (SEQ ID NO: 21) mRNA purification kit (Takara Bio Inc., Japan) to obtain polyA(+) RNA. .. This polyA(+) RNA (5 μg) was used as a template to prepare a cDNA library using a Lambda ZAP cDNA synthesis kit (cDNA synthesis kit/Uni ZAP XR vector kit/GigaPacl III Gold Packaging Extract (Agilent)) in accordance with the method recommend by the manufacturer. ..
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