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lambda zap ii vector dna  (Agilent technologies)


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    Structured Review

    Agilent technologies lambda zap ii vector dna
    Lambda Zap Ii Vector Dna, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda+vector+dna/us06844178-155-16-23
    Average 90 stars, based on 1 article reviews
    lambda zap ii vector dna - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Aphid biology: Expressed genes from alate Toxoptera citricida , the brown citrus aphid
    Article Snippet: A directional cDNA library was constructed in Lambda Uni-ZAP ® XR Vector using Stratagene's ZAP-cDNA Synthesis Kit (Stratagene, www.stratagene.com ).

    Article Title: Epistasis as a Determinant of the HIV-1 Protease's Robustness to Mutation
    Article Snippet: The resulting PCR products were digested with EcoRI and XhoI, isolated, and ligated to lambda DNA (Uni-ZAP XR Vector Kit, Stratagene).

    Article Title: Production of l -lactic acid by the yeast Candida sonorensis expressing heterologous bacterial and fungal lactate dehydrogenases
    Article Snippet: The genomic library of C. sonorensis ATCC32109 was prepared using partially Sau 3A digested size fractionated genomic DNA that was cloned into the Bam HI digested lambda DASHTM vector (Stratagene, La Jolla, CA, USA) as described previously [ ].

    Article Title: Corn plant event MON87460 and compositions and methods for detection thereof
    Article Snippet: This digested and purified genomic DNA was used for ligation into the Lambda DASH® II/EcoRI Vector Kit (Stratagene).

    Clone Assay:

    Article Title: Regulation of 7S globulin gene expression in zygotic and somatic embryos of oil palm.
    Article Snippet: We describe here the characterization and expression analysis accumulation in somatic embryos is mirrored by a low accuof the oil palm GLO7A gene encoding a 7S globulin protein. mulation of the GLO7A mRNA.. The in vitro production of 7S globulins (and more generally salt-soluble proteins) is imPrevious work carried out in our laboratory showed that 7S proved by the addition to the culture medium of arginine, globulins accumulate in the oil palm zygotic embryo mostly between the 14 and 17 weeks after pollination.. To investigate sucrose and ABA, the effects of these 3 components being additive.

    Isolation:

    Article Title: Regulation of 7S globulin gene expression in zygotic and somatic embryos of oil palm.
    Article Snippet: We describe here the characterization and expression analysis accumulation in somatic embryos is mirrored by a low accuof the oil palm GLO7A gene encoding a 7S globulin protein. mulation of the GLO7A mRNA.. The in vitro production of 7S globulins (and more generally salt-soluble proteins) is imPrevious work carried out in our laboratory showed that 7S proved by the addition to the culture medium of arginine, globulins accumulate in the oil palm zygotic embryo mostly between the 14 and 17 weeks after pollination.. To investigate sucrose and ABA, the effects of these 3 components being additive.

    Sequencing:

    Article Title: Regulation of 7S globulin gene expression in zygotic and somatic embryos of oil palm.
    Article Snippet: We describe here the characterization and expression analysis accumulation in somatic embryos is mirrored by a low accuof the oil palm GLO7A gene encoding a 7S globulin protein. mulation of the GLO7A mRNA.. The in vitro production of 7S globulins (and more generally salt-soluble proteins) is imPrevious work carried out in our laboratory showed that 7S proved by the addition to the culture medium of arginine, globulins accumulate in the oil palm zygotic embryo mostly between the 14 and 17 weeks after pollination.. To investigate sucrose and ABA, the effects of these 3 components being additive.

    cDNA Library Assay:

    Article Title: Regulation of 7S globulin gene expression in zygotic and somatic embryos of oil palm.
    Article Snippet: We describe here the characterization and expression analysis accumulation in somatic embryos is mirrored by a low accuof the oil palm GLO7A gene encoding a 7S globulin protein. mulation of the GLO7A mRNA.. The in vitro production of 7S globulins (and more generally salt-soluble proteins) is imPrevious work carried out in our laboratory showed that 7S proved by the addition to the culture medium of arginine, globulins accumulate in the oil palm zygotic embryo mostly between the 14 and 17 weeks after pollination.. To investigate sucrose and ABA, the effects of these 3 components being additive.

    Article Title: Monoterpene glycosyltransferase originating from hop and method for using same
    Article Snippet: From immature and mature cones and leaves of Humulus lupulus (Shinshu wase variety), RNA was extracted with a Spectrum Plant Total RNA Kit (SIGMA), followed by treatment with an Oligotex-dT30 (SEQ ID NO: 21) mRNA purification kit (Takara Bio Inc., Japan) to obtain polyA(+) RNA. .. This polyA(+) RNA (5 μg) was used as a template to prepare a cDNA library using a Lambda ZAP cDNA synthesis kit (cDNA synthesis kit/Uni ZAP XR vector kit/GigaPacl III Gold Packaging Extract (Agilent)) in accordance with the method recommend by the manufacturer. ..

    Construct:

    Article Title: Regulation of 7S globulin gene expression in zygotic and somatic embryos of oil palm.
    Article Snippet: We describe here the characterization and expression analysis accumulation in somatic embryos is mirrored by a low accuof the oil palm GLO7A gene encoding a 7S globulin protein. mulation of the GLO7A mRNA.. The in vitro production of 7S globulins (and more generally salt-soluble proteins) is imPrevious work carried out in our laboratory showed that 7S proved by the addition to the culture medium of arginine, globulins accumulate in the oil palm zygotic embryo mostly between the 14 and 17 weeks after pollination.. To investigate sucrose and ABA, the effects of these 3 components being additive.

    Cloning:

    Article Title: Vectors for production of antibodies
    Article Snippet: .. Bp 717-673 Bp 3745-4184 Multiple cloning site from pBluescriptII sk(−), from the XhoI site bp 672-235 Bp 4190-4259 70 bp of IS10 from Tn10 Bp 4260-4301 Lambda DNA from pNK2859 Bp 4302-5167 Non-coding DNA from pNK2859 Bp 5168-7368 pBluescriptII sk(−) base vector (Stratagene, INC) bp 761-2961 SEQ ID NO: 2 X-MCS (Vector #5005) pTNMCS (Base Vector) with MCS Extension Bp 1-132 Remainder of F1 (−) ori of pBluescriptII sk(−) (Stratagene) Bp 4-135 Bp 133-1785 CMV Promoter/Enhancer from vector pGWIZ (Gene Therapy Systems) Bp 1786-3018 Transposase, modified from Tn10 (GeneBank accession #J01829) Bp 81-1313 Bp 3019-3021 Engineered stop codon Bp 3022-3374 Non-coding DNA from vector pNK2859 Bp 3375-3416 Lambda DNA from pNK2859 Bp 3417-3486 70 bp of IS10 left from Tn10 (GeneBank accession #J01829 Bp 1-70) Bp 3487-3704 Multiple cloning site from pBluescriptII sk(−), thru XmaI Bp 3705-3749 Multiple cloning site from pBluescriptII sk(−), from XmaI thru XhoI Bp 3750-3845 Multiple cloning site extension from XhoI thru PspOMI BP 3846-4275 Multiple cloning site from pBluescriptII sk(−), from PspOMI Bp 4276-4345 70 bp of IS10 from Tn10 (GeneBank accession #J01829 Bp 70-1) Bp 4346-4387 Lambda DNA from pNK2859 Bp 4388-5254 Non-coding DNA from pNK2859 Bp 5255-7455 pBluescriptII sk(−) base vector (Stratagene, INC) Bp 761-2961 SEQ ID NO: 3 HS4 Flanked BV (Vector #5006) Bp 1-132 Remainder of F1 (−) ori of pBluescriptII sk(−) (Stratagene) Bp 4-135 Bp 133-1785 CMV Promoter/Enhancer from vector pGWIZ (Gene Therapy Systems) Bp 229-1873, including the combination of 2 NruI cut sites Bp 1786-3018 Transposase, modified from Tn10 (GeneBank accession #J01829) Bp 81-1313 Bp 3019-3021 Engineered stop codon Bp 3022-3374 Non-coding DNA from vector pNK2859 Bp 3375-3416 Lambda DNA from pNK2859 Bp 3417-3490 70 bp of IS10 left from Tn10 (GeneBank accession #J01829 Bp 1-70) Bp 3491-3680 Multiple cloning site from pBluescriptII sk(−), thru NotI Bp 926-737 Bp 3681-4922 HS4—Beta-globin Insulator Element from Chicken gDNA Bp 4923-5018 Multiple cloning site extension XhoI thru MluI Bp 5019-6272 HS4—Beta-globin Insulator Element from Chicken gDNA Bp 6273-6342 70 bp of IS10 from Tn10 (GeneBank accession #J01829 Bp 70-1) Bp 6343-6389 Lambda DNA from pNK2859 Bp 6390-8590 pBluescriptII sk(−) base vector (Stratagene, INC) Bp 761-2961 SEQ ID NO: 4 K Tn-10 HS4 Flanked Backbone (Vector #5012) Bp. .. 1-132 Remaining of F1 (−) Ori from pBluescript II sk(−) (Stratagene Bp 4-135).

    Lambda DNA Preparation:

    Article Title: Vectors for production of antibodies
    Article Snippet: .. Bp 717-673 Bp 3745-4184 Multiple cloning site from pBluescriptII sk(−), from the XhoI site bp 672-235 Bp 4190-4259 70 bp of IS10 from Tn10 Bp 4260-4301 Lambda DNA from pNK2859 Bp 4302-5167 Non-coding DNA from pNK2859 Bp 5168-7368 pBluescriptII sk(−) base vector (Stratagene, INC) bp 761-2961 SEQ ID NO: 2 X-MCS (Vector #5005) pTNMCS (Base Vector) with MCS Extension Bp 1-132 Remainder of F1 (−) ori of pBluescriptII sk(−) (Stratagene) Bp 4-135 Bp 133-1785 CMV Promoter/Enhancer from vector pGWIZ (Gene Therapy Systems) Bp 1786-3018 Transposase, modified from Tn10 (GeneBank accession #J01829) Bp 81-1313 Bp 3019-3021 Engineered stop codon Bp 3022-3374 Non-coding DNA from vector pNK2859 Bp 3375-3416 Lambda DNA from pNK2859 Bp 3417-3486 70 bp of IS10 left from Tn10 (GeneBank accession #J01829 Bp 1-70) Bp 3487-3704 Multiple cloning site from pBluescriptII sk(−), thru XmaI Bp 3705-3749 Multiple cloning site from pBluescriptII sk(−), from XmaI thru XhoI Bp 3750-3845 Multiple cloning site extension from XhoI thru PspOMI BP 3846-4275 Multiple cloning site from pBluescriptII sk(−), from PspOMI Bp 4276-4345 70 bp of IS10 from Tn10 (GeneBank accession #J01829 Bp 70-1) Bp 4346-4387 Lambda DNA from pNK2859 Bp 4388-5254 Non-coding DNA from pNK2859 Bp 5255-7455 pBluescriptII sk(−) base vector (Stratagene, INC) Bp 761-2961 SEQ ID NO: 3 HS4 Flanked BV (Vector #5006) Bp 1-132 Remainder of F1 (−) ori of pBluescriptII sk(−) (Stratagene) Bp 4-135 Bp 133-1785 CMV Promoter/Enhancer from vector pGWIZ (Gene Therapy Systems) Bp 229-1873, including the combination of 2 NruI cut sites Bp 1786-3018 Transposase, modified from Tn10 (GeneBank accession #J01829) Bp 81-1313 Bp 3019-3021 Engineered stop codon Bp 3022-3374 Non-coding DNA from vector pNK2859 Bp 3375-3416 Lambda DNA from pNK2859 Bp 3417-3490 70 bp of IS10 left from Tn10 (GeneBank accession #J01829 Bp 1-70) Bp 3491-3680 Multiple cloning site from pBluescriptII sk(−), thru NotI Bp 926-737 Bp 3681-4922 HS4—Beta-globin Insulator Element from Chicken gDNA Bp 4923-5018 Multiple cloning site extension XhoI thru MluI Bp 5019-6272 HS4—Beta-globin Insulator Element from Chicken gDNA Bp 6273-6342 70 bp of IS10 from Tn10 (GeneBank accession #J01829 Bp 70-1) Bp 6343-6389 Lambda DNA from pNK2859 Bp 6390-8590 pBluescriptII sk(−) base vector (Stratagene, INC) Bp 761-2961 SEQ ID NO: 4 K Tn-10 HS4 Flanked Backbone (Vector #5012) Bp. .. 1-132 Remaining of F1 (−) Ori from pBluescript II sk(−) (Stratagene Bp 4-135).

    Modification:

    Article Title: Vectors for production of antibodies
    Article Snippet: .. Bp 717-673 Bp 3745-4184 Multiple cloning site from pBluescriptII sk(−), from the XhoI site bp 672-235 Bp 4190-4259 70 bp of IS10 from Tn10 Bp 4260-4301 Lambda DNA from pNK2859 Bp 4302-5167 Non-coding DNA from pNK2859 Bp 5168-7368 pBluescriptII sk(−) base vector (Stratagene, INC) bp 761-2961 SEQ ID NO: 2 X-MCS (Vector #5005) pTNMCS (Base Vector) with MCS Extension Bp 1-132 Remainder of F1 (−) ori of pBluescriptII sk(−) (Stratagene) Bp 4-135 Bp 133-1785 CMV Promoter/Enhancer from vector pGWIZ (Gene Therapy Systems) Bp 1786-3018 Transposase, modified from Tn10 (GeneBank accession #J01829) Bp 81-1313 Bp 3019-3021 Engineered stop codon Bp 3022-3374 Non-coding DNA from vector pNK2859 Bp 3375-3416 Lambda DNA from pNK2859 Bp 3417-3486 70 bp of IS10 left from Tn10 (GeneBank accession #J01829 Bp 1-70) Bp 3487-3704 Multiple cloning site from pBluescriptII sk(−), thru XmaI Bp 3705-3749 Multiple cloning site from pBluescriptII sk(−), from XmaI thru XhoI Bp 3750-3845 Multiple cloning site extension from XhoI thru PspOMI BP 3846-4275 Multiple cloning site from pBluescriptII sk(−), from PspOMI Bp 4276-4345 70 bp of IS10 from Tn10 (GeneBank accession #J01829 Bp 70-1) Bp 4346-4387 Lambda DNA from pNK2859 Bp 4388-5254 Non-coding DNA from pNK2859 Bp 5255-7455 pBluescriptII sk(−) base vector (Stratagene, INC) Bp 761-2961 SEQ ID NO: 3 HS4 Flanked BV (Vector #5006) Bp 1-132 Remainder of F1 (−) ori of pBluescriptII sk(−) (Stratagene) Bp 4-135 Bp 133-1785 CMV Promoter/Enhancer from vector pGWIZ (Gene Therapy Systems) Bp 229-1873, including the combination of 2 NruI cut sites Bp 1786-3018 Transposase, modified from Tn10 (GeneBank accession #J01829) Bp 81-1313 Bp 3019-3021 Engineered stop codon Bp 3022-3374 Non-coding DNA from vector pNK2859 Bp 3375-3416 Lambda DNA from pNK2859 Bp 3417-3490 70 bp of IS10 left from Tn10 (GeneBank accession #J01829 Bp 1-70) Bp 3491-3680 Multiple cloning site from pBluescriptII sk(−), thru NotI Bp 926-737 Bp 3681-4922 HS4—Beta-globin Insulator Element from Chicken gDNA Bp 4923-5018 Multiple cloning site extension XhoI thru MluI Bp 5019-6272 HS4—Beta-globin Insulator Element from Chicken gDNA Bp 6273-6342 70 bp of IS10 from Tn10 (GeneBank accession #J01829 Bp 70-1) Bp 6343-6389 Lambda DNA from pNK2859 Bp 6390-8590 pBluescriptII sk(−) base vector (Stratagene, INC) Bp 761-2961 SEQ ID NO: 4 K Tn-10 HS4 Flanked Backbone (Vector #5012) Bp. .. 1-132 Remaining of F1 (−) Ori from pBluescript II sk(−) (Stratagene Bp 4-135).

    cDNA Synthesis:

    Article Title: Monoterpene glycosyltransferase originating from hop and method for using same
    Article Snippet: From immature and mature cones and leaves of Humulus lupulus (Shinshu wase variety), RNA was extracted with a Spectrum Plant Total RNA Kit (SIGMA), followed by treatment with an Oligotex-dT30 (SEQ ID NO: 21) mRNA purification kit (Takara Bio Inc., Japan) to obtain polyA(+) RNA. .. This polyA(+) RNA (5 μg) was used as a template to prepare a cDNA library using a Lambda ZAP cDNA synthesis kit (cDNA synthesis kit/Uni ZAP XR vector kit/GigaPacl III Gold Packaging Extract (Agilent)) in accordance with the method recommend by the manufacturer. ..



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    Image Search Results


    Eap binding to DNA is affected by the state and type of DNA. Eap (0.5 μg/ml) was co-incubated with DNA samples (100 ng/ml) obtained from different sources, and subsequently attached to OTS silicon prior to AFM imaging. Representative AFM height images of Eap/DNA complexes adhering to OTS silicon from three independent experiments are shown: (A) Phage lambda DNA. (B) Artificial 1.4 kb PCR DNA-product. (C) Pst I-digested plasmid pBR322 (D) Circular pBR322 isolated from E. coli DH5α. (E) Sheared herring sperm DNA. Eap molecules putatively adhering to DNA (white arrows), OTS (yellow arrows), or cross-linking DNA (green arrows) are indicated. DNA molecules displayed in (A–C) , and (E) were aligned to OTS silicon by DNA combing.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: The Staphylococcus aureus Extracellular Adherence Protein Eap Is a DNA Binding Protein Capable of Blocking Neutrophil Extracellular Trap Formation

    doi: 10.3389/fcimb.2018.00235

    Figure Lengend Snippet: Eap binding to DNA is affected by the state and type of DNA. Eap (0.5 μg/ml) was co-incubated with DNA samples (100 ng/ml) obtained from different sources, and subsequently attached to OTS silicon prior to AFM imaging. Representative AFM height images of Eap/DNA complexes adhering to OTS silicon from three independent experiments are shown: (A) Phage lambda DNA. (B) Artificial 1.4 kb PCR DNA-product. (C) Pst I-digested plasmid pBR322 (D) Circular pBR322 isolated from E. coli DH5α. (E) Sheared herring sperm DNA. Eap molecules putatively adhering to DNA (white arrows), OTS (yellow arrows), or cross-linking DNA (green arrows) are indicated. DNA molecules displayed in (A–C) , and (E) were aligned to OTS silicon by DNA combing.

    Article Snippet: Herring sperm DNA (10164142), phage lambda DNA (D3779), and pBR322 (N3033L) DNA were purchased from Invitrogen (Karlsruhe, Germany), Sigma-Aldrich, and New England Biolabs (Frankfurt, Germany), respectively.

    Techniques: Binding Assay, Incubation, Imaging, Lambda DNA Preparation, Plasmid Preparation, Isolation